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Evaluation of a Novel Medium for Screening Specimens from Hospitalized Patients To Detect Methicillin-Resistant Staphylococcus aureus

机译：新型媒介物从住院患者中筛选标本以检测耐甲氧西林金黄色葡萄球菌的评估

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A novel medium, Oxacillin Resistant Screening Agar (ORSA) medium, was evaluated for the screening of specimens for methicillin-resistant Staphylococcus aureus (MRSA) in the hospital setting. Screening swabs (swabs of the nose, throat, perineum, and infected sites) were inoculated onto the new ORSA medium and into an enrichment broth (Muller-Hinton broth supplemented with NaCl and oxacillin). After 24 h of incubation, the enrichment broth was subcultured onto one ORSA plate and one lipovitellin Chapman salt agar plate. The sensitivities for the detection of MRSA were calculated for each medium alone and for the media in combination. A low sensitivity (74%) was obtained when ORSA medium was used alone as a primary culture, whereas the sensitivity was 88% when a single selective enrichment broth was used. Among the 414 blue colonies observed on ORSA plates, only 47% were found to be MRSA, 40% were coagulase-negative staphylococci, 7% were Enterococcus species, and 2% were methicillin-sensitive S. aureus. The optimal incubation time for the ORSA plates was evaluated. On primary culture, 38% of the blue MRSA colonies were visible only after 48 h of incubation (no blue colonies were not seen after 24 h of incubation), whereas 94% of the colonies were already visible at 24 h when ORSA plates were used for subcultures. In conclusion, the advantage of the novel ORSA medium is the ease of recognition of mannitol-fermenting bacteria, but further identification tests are needed to confirm the identification of S. aureus. An enrichment broth is still needed to ensure a good sensitivity for the recovery of MRSA, and an incubation time of 48 h is required for primary culture on ORSA medium.
机译：在医院环境中，评估了一种新型抗氧西林筛选琼脂（ORSA）培养基的标本筛选，以筛选耐甲氧西林金黄色葡萄球菌（MRSA）的标本。将筛选的拭子（鼻，咽喉，会阴和感染部位）接种到新的ORSA培养基上，并接种到富集肉汤（补充有NaCl和奥沙西林的Muller-Hinton肉汤）中。孵育24小时后，将富集肉汤传代培养到1个ORSA平板和1个lipovitellin查普曼盐琼脂平板上。分别针对每种培养基和组合培养基计算了检测MRSA的灵敏度。当单独使用ORSA培养基作为原代培养物时，灵敏度低（74％），而使用单个选择性富集肉汤时灵敏度为88％。在ORSA平板上观察到的414个蓝色菌落中，只有47％被发现为MRSA，凝固酶阴性葡萄球菌为40％，肠球菌为7％，对甲氧西林敏感的金黄色葡萄球菌为2％。评估了ORSA板的最佳孵育时间。在原代培养中，仅在孵育48小时后可见38％的蓝色MRSA菌落（孵育24小时后未见蓝色菌落），而使用ORSA平板在24 h时已经可见94％的菌落对于亚文化。总之，新型ORSA培养基的优点是易于识别甘露醇发酵细菌，但还需要进一步的鉴定测试以确认金黄色葡萄球菌的鉴定。为了确保对MRSA的恢复具有良好的敏感性，仍然需要一个浓缩肉汤，在ORSA培养基上进行初次培养需要48小时的孵育时间。

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